CGC Bibliography Paper 5323

A liquid-based method for the assessment of bacterial pathogenicity using the nematode Caenorhabditis elegans.

Smith MP, Laws TR, Atkins TP, Oyston PCF, de Pomerai DI, Titball RW

Medline:

12044672

Citation:

FEMS Microbiology Letters 210: 181-185 2002

Type:

ARTICLE

Genes:

phm-2

Abstract:

Caenorhabditis elegans has previously been used as an alternative to mammalian models of infection with bacterial pathogens. We have developed a liquid-based assay to measure the effect of bacteria on the feeding ability of C elegans. Using this assay we have shown that Pseudomonas aeruginosa strain PA14, Burkholderia pseudomallei and Yersinia pestis were able to inhibit feeding of C elegans strain N2. An increase in sensitivity of the assay was achieved by using C elegans mutant phm-2, in place of the wild-type strain. Using this assay, P. aeruginosa PA01 inhibited the feeding of C elegans mutant phm-2. Such liquid-based feeding assays are ideally suited to the high-throughput screening of mutants of bacterial