CGC Bibliography Paper 5306

Characterization of the chondroitin sulfates in wild type Caenorhabditis elegans.

Beeber C, Kieras FJ

Medline:
12054666
Citation:
Biochemical and Biophysical Research Communications 293: 1374-1376 2002
Type:
ARTICLE
Genes:
Abstract:
The purpose of this study was to isolate and characterize the GAGs from the wild type nematode Caenorhabditis elegans in preparation for the characterization of the transgenic form constructed by Link [Proc. Natl. Acad. Sci. USA 92 (1995) 9368] which expresses various forms of P-peptide (or A4 peptide). This peptide forms deposits very similar to the ones found in the neuritic plaques and neurofibrillary tangles in Alzheimer disease (AD). Characterization has been accomplished by degradation with specific enzymes and analysis of the products by TLC and HPLC. The results were compared with earlier works and shown to differ in disaccharide content.