CGC Bibliography Paper 5194

Isolation of the interacting molecules with GEX-3 by a novel functional screening.

Tsuboi D, Qadota H, Kasuya K, Amano M, Kaibuchi K

Medline:

11922622

Citation:

Biochemical and Biophysical Research Communications 292: 697-701 2002

Type:

ARTICLE

Genes:

dyn-1 emb-9 gei-1 gei-2 gei-3 gei-4 gei-5 gei-6 gei-7 gei-8 gei-9 gei-10 gei-11 gei-12 gei-13 gei-14 gei-15 gei-16 gei-17 gei-18 gei-19 gei-20 gei-21 gei-22 gex-2 gex-3 lin-13 lin-26 puf-6 zpf-1

Abstract:

To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional