CGC Bibliography Paper 5172
Lamin-dependent localization of UNC-84, a protein required for nuclear migration in Caenorhabditis elegans.
Lee KK,
Starr D,
Cohen M,
Liu J,
Han M,
Wilson KL,
Gruenbaum Y
- Medline:
- 11907270
- Citation:
- Molecular Biology of the Cell 13: 892-901 2002
- Type:
- ARTICLE
- Genes:
- anc-1 unc-83 unc-84
- Abstract:
- Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear periphery until late anaphase, similar to known inner nuclear membrane proteins. UNC-84 protein is first detected at the 26-cell stage and thereafter is present in most cells during development and in adults. UNC-84 is properly expressed in unc-83 and anc-1 lines, which have phenotypes similar to unc-84, suggesting that neither the expression nor nuclear envelope localization of UNC-84 depends on UNC-83 or ANC-1 proteins. The envelope localization of Ce-lamin, Ce-emerin, Ce-MAN1, and nucleoporins are unaffected by the loss of UNC-84. UNC-84 is not required for centrosome attachment to the nucleus because centrosomes are localized normally in unc-84 hyp7 cells despite a nuclear migration defect. Models for UNC-84 localization are discussed.