CGC Bibliography Paper 5150

The G protein gamma subunit gpc-1 of the nematode C. elegans is involved in taste adaptation.

Jansen G, Weinkove D, Plasterk RHA

Medline:

11867526

Citation:

EMBO Journal 21: 986-994 2002

Type:

ARTICLE

Genes:

adp-1gpc-1 gpc-2 osm-9

Abstract:

Caenorhabditis elegans has two heterotrimeric G-protein gamma subunits, gpc-1 and gpc-2. Although GPC-1 is specifically expressed in sensory neurons, it is not essential for the detection of odorants or salts. To test whether GPC-1 is involved in sensory plasticity, we developed a water soluble compound adaptation assay. The behaviour of wild-type animals in this assay confirms that prolonged exposure to salts can abolish chemo-attraction to these compounds. This process is time and concentration dependent, partly salt specific and reversible. In contrast, gpc-1 mutant animals show clear deficits in their ability to adapt to NaAc, NaCl and NH4Cl, but normal wild-type adaptation to odorants. Two other loci previously implicated in odorant adaptation, adp-1 and osm-9, are also involved in adaptation to salts. Our finding that G proteins, OSM-9 and ADP-1 are involved in taste adaptation offer the first molecular insight into this process.