CGC Bibliography Paper 3097

Identification of N-terminal minimal transactivation domain of CBP, p300 and Caenorhabditis elegans homologues.

Yoshida E, Nakajima T, Murakami K, Fukamizu A

Medline:

98201627

Citation:

Gene 208: 307-314 1998

Type:

ARTICLE

Genes:

Abstract:

CBP/p300 is a multidomain transcriptional cofactor that acts in junction with other factors to regulate transcription. To elucidate the domain function of CBP, we fused its dissected fragments to Gal4 DNA-binding domain and transfected the deletion mutants into several lines. First, we found that the minimal transactivation domain (MTD) at the N-terminal portion maps to between 344 and 451 aa, and shows activity in a cell-type dependent manner. Second, we cloned C. elegans homologues corresponding to the MTD by RT-PCR and identified the three related products, two of which exhibited weak transcriptional activity. Finally, by means of the yeast two hybrid screening using MTD as a bait, we cloned hypoxia-inducible factor (HIF) 1a and Stat2 cDNAs. These results suggested a functional role of MTD located at the N-terminal region of CBP/p300 in connecting to transcriptional factors.