CGC Bibliography Paper 3066

Molecular analysis of the tpa-1 gene and its role in the development and behavior of Caenorhabditis elegans.

Tabuse Y, Sano T, Nishiwaki K, Miwa J

Medline:

Citation:

NEC Research & Development 39: 118-126 1998

Type:

ARTICLE

Genes:

tpa-1

Abstract:

Protein kinase C (PKC) is now known to be a key enzyme in intracellular signaling pathways which mediate various physiological functions in diverse eukaryotic organisms from yeast to humans. The tpa-1 gene was identified as a target of tumor-promoting phorbol esters; which cause developmental and behavioral defects in the nematode Caenorhabditis elegans (C. elegans). Molecular cloning of the tpa-l gene revealed that it encodes two PKC isoforms, TPA-1A and TPA-1B. Further molecular genetic analysis showed that the tpa-l gene product is necessary for C. elegans to be sensitive to phorbol esters. This report reviews the identification and molecular analyses of the tpa-l gene of C. elegans.